8SIP
Structure of a mouse IgG antibody fragment that binds Inosine, an RNA modification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-31 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.817, 49.090, 57.385 |
| Unit cell angles | 83.84, 88.82, 89.68 |
Refinement procedure
| Resolution | 57.040 - 1.940 |
| R-factor | 0.2064 |
| Rwork | 0.204 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.099 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.040 | 2.009 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.190 | 1.506 |
| Rmeas | 0.203 | 1.642 |
| Rpim | 0.069 | 0.643 |
| Number of reflections | 30888 | 2931 |
| <I/σ(I)> | 6.33 | 3.17 |
| Completeness [%] | 96.2 | 91.25 |
| Redundancy | 7.1 | 4.9 |
| CC(1/2) | 0.991 | 0.586 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 293 | 50 mM TRIS PH 8.3, 15% PEG 4000, 0.1 mM EDTA |
