8SIP

Structure of a mouse IgG antibody fragment that binds Inosine, an RNA modification

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	39.817, 49.090, 57.385
Unit cell angles	83.84, 88.82, 89.68

Refinement procedure

Resolution	57.040 - 1.940
R-factor	0.2064
Rwork	0.204
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.099
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.040	2.009
High resolution limit [Å]	1.940	1.940
Rmerge	0.190	1.506
Rmeas	0.203	1.642
Rpim	0.069	0.643
Number of reflections	30888	2931
<I/σ(I)>	6.33	3.17
Completeness [%]	96.2	91.25
Redundancy	7.1	4.9
CC(1/2)	0.991	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	293	50 mM TRIS PH 8.3, 15% PEG 4000, 0.1 mM EDTA