8SHR

Crystal Structure of PRMT3 with Compound YD1-214

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	70.211, 70.211, 175.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.700 - 1.920
R-factor	0.18561
Rwork	0.185
R-free	0.21491
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.335
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.920	1.920
Rmerge	0.063	0.985
Number of reflections	34468	1702
<I/σ(I)>	9.7	2.5
Completeness [%]	100.0	100
Redundancy	18.1	17.6
CC(1/2)	1.000	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	2.4 M Sodium malonate pH 7.0