8SH5
Crystal structure of 3'cap-independent translation enhancers (CITE) from Pea enation mosaic virus RNA 2 (PEMV2) with Fab BL3-6K170A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979180 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.346, 65.460, 89.817 |
Unit cell angles | 90.00, 110.29, 90.00 |
Refinement procedure
Resolution | 59.420 - 2.750 |
R-factor | 0.2554 |
Rwork | 0.251 |
R-free | 0.29660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.578 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.420 | 2.848 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.191 | 0.572 |
Rmeas | 0.219 | |
Rpim | 0.104 | |
Number of reflections | 18060 | 1760 |
<I/σ(I)> | 14.45 | |
Completeness [%] | 97.3 | 97.01 |
Redundancy | 3.8 | 3.7 |
CC(1/2) | 0.979 | 0.793 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 5 mM MgCl2, 50 mM HEPES pH 7.0, 25% PEG 550, 2% Benzamidine hydrochloride |