8SGG
Crystal structure of Cy137D09, a monoclonal antibody isolated from macaques immunized with an Epstein-Barr virus glycoprotein 350 (gp350) nanoparticle vaccine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-18 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.794, 71.463, 86.451 |
Unit cell angles | 90.00, 107.25, 90.00 |
Refinement procedure
Resolution | 36.120 - 2.030 |
R-factor | 0.1926 |
Rwork | 0.191 |
R-free | 0.24650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8sgn |
RMSD bond length | 0.006 |
RMSD bond angle | 0.876 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmeas | 0.171 | 0.722 |
Number of reflections | 51917 | 2870 |
<I/σ(I)> | 9.4 | |
Completeness [%] | 85.7 | |
Redundancy | 2.7 | |
CC(1/2) | 0.642 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M calcium acetate, 0.1M sodium cacodylate pH 6.5, 17.5% w/v PEG 8000 |