8SG7
Adeno-Associated Virus Bat origin capsid protein basic regions in complex with importin-alpha 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.065, 90.556, 99.816 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.400 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.935 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.111 | 1.335 |
| Rpim | 0.051 | 0.614 |
| Number of reflections | 32506 | 3158 |
| <I/σ(I)> | 8.8 | 2 |
| Completeness [%] | 99.9 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.75M Na Citrate, 0.01M DTT, 0.1M HEPES, pH7.5 |
