8SFU
Crystal structure of TuUGT202A2 (Tetur22g00270) in complex with naringin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.482, 115.677, 165.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.402 - 1.800 |
| Rwork | 0.180 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.671 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.093 | 0.843 |
| Rpim | 0.035 | 0.301 |
| Number of reflections | 83547 | 4097 |
| <I/σ(I)> | 27.7 | 1.9 |
| Completeness [%] | 97.4 | 96.4 |
| Redundancy | 6.7 | 6.9 |
| CC(1/2) | 0.992 | 0.894 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Protein incubated with 1mM UDP and 2.5 mM naringin 0.2 M Sodium Chloride 0.1 M BIS-TRIS pH 6.5 25% w/v Polyethylene glycol 3,350 |
