8SEF

Crystal structure of Cy137C02, a monoclonal antibody isolated from macaques immunized with an Epstein-Barr virus glycoprotein 350 (gp350) nanoparticle vaccine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	145.389, 67.125, 50.244
Unit cell angles	90.00, 106.01, 90.00

Refinement procedure

Resolution	38.270 - 2.470
R-factor	0.2266
Rwork	0.222
R-free	0.26350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8sic
RMSD bond length	0.002
RMSD bond angle	0.620
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.560
High resolution limit [Å]	2.470	2.470
Rmeas	0.132
Number of reflections	16004	1453
<I/σ(I)>	11.1
Completeness [%]	95.2
Redundancy	2.9
CC(1/2)		0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	1x PBS, Cryoprotectant: 3M ammonium sulfate, 10% glycerol