8SDW
Crystal structure of the non-myristoylated mutant [L8K]Arf1 in complex with a GDP analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.654, 55.015, 78.472 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.240 - 1.750 |
Rwork | 0.190 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20rc3-4406) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.780 |
High resolution limit [Å] | 1.750 | 4.750 | 1.750 |
Rpim | 0.025 | ||
Number of reflections | 18613 | 1035 | 819 |
<I/σ(I)> | 27.1 | ||
Completeness [%] | 98.7 | ||
Redundancy | 6.2 | ||
CC(1/2) | 0.996 | 0.997 | 0.976 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277.15 | 0.2 M Sodium nitrate, 20% w/v Polyethylene glycol 3,350, pH 6.8 |