8SDJ

Hydrophobic Interactions Drive the Tetrameric Assembly of the TRIM20 Coiled-Coil Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	38.600, 38.830, 164.300
Unit cell angles	90.00, 89.94, 90.00

Refinement procedure

Resolution	9.000 - 2.400
Rwork	0.259
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.702
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.033	0.169
Number of reflections	18553	2593
<I/σ(I)>	13.6	4
Completeness [%]	95.7	94.3
Redundancy	2.9	2.7
CC(1/2)	0.999	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	298	0.1M HEPES pH8.0, 35% 1,2-Propanediol, 10% PEG400