8SDI
Hydrophobic Interactions Drive the Tetrameric Assembly of the TRIM45 Coiled-Coil Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 41 |
| Unit cell lengths | 55.850, 55.850, 347.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 11.985 - 2.700 |
| Rwork | 0.239 |
| R-free | 0.27070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.645 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 11.985 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.037 | 0.267 |
| Number of reflections | 14141 | 2100 |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 97.6 | 98.7 |
| Redundancy | 4.1 | 3.9 |
| CC(1/2) | 1.000 | 0.978 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 0.2M Ammonium dihydrogen phosphate, 35% (v/v) glycerol |
