8SDE
Electrostatic-Mediated Tetramerization of the Coiled-Coil Domain in TRIM29
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 45.050, 47.630, 107.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.981 - 2.270 |
| Rwork | 0.227 |
| R-free | 0.27510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.785 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.390 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.071 | 0.319 |
| Number of reflections | 5400 | 782 |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 96.9 | 98.8 |
| Redundancy | 9.1 | 8.7 |
| CC(1/2) | 0.999 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.7 | 295 | 10% glycerol, 38% MPD, and 0.1M Acetic acid pH4.7 |
