8SDB
Crystal Structure of E.Coli Branching Enzyme in complex with malto-octose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-19 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9785 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 146.489, 146.489, 294.920 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.850 - 3.000 |
R-factor | 0.2189 |
Rwork | 0.214 |
R-free | 0.31540 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.356 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.850 | 3.107 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 71397 | 5917 |
<I/σ(I)> | 12.46 | 2.55 |
Completeness [%] | 96.2 | |
Redundancy | 3.2 | |
CC(1/2) | 0.850 | 0.800 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.3 M ammonium tartrate, pH 7.6, 10% PEG 4k |