8SDB

Crystal Structure of E.Coli Branching Enzyme in complex with malto-octose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-19
Detector	MAR CCD 130 mm
Wavelength(s)	0.9785
Spacegroup name	P 32 2 1
Unit cell lengths	146.489, 146.489, 294.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.850 - 3.000
R-factor	0.2189
Rwork	0.214
R-free	0.31540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.356
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.850	3.107
High resolution limit [Å]	3.000	3.000
Number of reflections	71397	5917
<I/σ(I)>	12.46	2.55
Completeness [%]	96.2
Redundancy	3.2
CC(1/2)	0.850	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.3 M ammonium tartrate, pH 7.6, 10% PEG 4k