8SCY

Lysozyme crystallized in cyclic olefin copolymer-based microfluidic chips

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	295
Detector technology	PIXEL
Collection date	2021-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979460
Spacegroup name	P 43 21 2
Unit cell lengths	79.148, 79.148, 38.048
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.570 - 1.450
R-factor	0.1877
Rwork	0.185
R-free	0.21160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.871
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.570	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.095	0.054
Rmeas	0.099	0.057
Rpim	0.026	0.015
Number of reflections	22046	1243
<I/σ(I)>	12.6
Completeness [%]	100.0	100
Redundancy	13.2	13.1
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	4.6	277	2 M NaCl with 0.1 M sodium acetate buffer (pH 4.6)