8SCC
Crystal Structure of L-galactose 1-dehydrogenase de Myrciaria dubia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS SIRIUS BEAMLINE MANACA |
| Synchrotron site | LNLS SIRIUS |
| Beamline | MANACA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-28 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.977180 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.310, 55.796, 107.732 |
| Unit cell angles | 90.00, 110.00, 90.00 |
Refinement procedure
| Resolution | 101.230 - 2.090 |
| R-factor | 0.1823 |
| Rwork | 0.180 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.856 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.230 | 2.200 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.239 | 0.912 |
| Rmeas | 0.273 | 1.049 |
| Rpim | 0.131 | 0.511 |
| Total number of observations | 168734 | 20450 |
| Number of reflections | 41096 | 5282 |
| <I/σ(I)> | 6.3 | 2.5 |
| Completeness [%] | 98.0 | |
| Redundancy | 4.1 | 3.9 |
| CC(1/2) | 0.982 | 0.662 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 0.5M Ammonium sulfate, 1.0M Lithium sulfate, 0.1M Sodium citrate |
