8S9M

DNA cytosine-N4 methyltransferase (residues 79-324) from the Bdelloid rotifer Adineta vaga

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	57.581, 69.096, 124.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.680 - 1.490
R-factor	0.1617
Rwork	0.160
R-free	0.19710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.783
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.680	1.540
High resolution limit [Å]	1.490	1.490
Rpim	0.084	0.780
Number of reflections	40906	4026
<I/σ(I)>	9.9	2.2
Completeness [%]	99.9	99.3
Redundancy	10.3	4.9
CC(1/2)	0.985	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2 M Potassium citrate tribasic monohydrate, 20% PEG 3350