8S96
RNase A-Adenosine 5'-Heptaphosphate (RNaseA.p7A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR-H |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2023-02-13 |
Detector | APEX II CCD |
Wavelength(s) | 1.544178 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 99.383, 31.347, 68.962 |
Unit cell angles | 90.00, 90.67, 90.00 |
Refinement procedure
Resolution | 34.480 - 1.680 |
R-factor | 0.2124 |
Rwork | 0.211 |
R-free | 0.23670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1afu |
RMSD bond length | 0.007 |
RMSD bond angle | 1.026 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.480 | 1.780 |
High resolution limit [Å] | 1.680 | 1.680 |
Rpim | 0.029 | 0.396 |
Number of reflections | 24657 | 3862 |
<I/σ(I)> | 3.55 | |
Completeness [%] | 99.9 | |
Redundancy | 10.43 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 25% PEG4000, 20 mM citrate, pH 6, crystal soaked in 50 mM ligand, 30% PEG4000, 25% glycerol, 20 mM citrate |