8S8V

OPR3 variant R283D in its monomeric form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-08
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.87
Spacegroup name	P 21 21 21
Unit cell lengths	86.390, 89.314, 95.528
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.660 - 3.000
R-factor	0.2545
Rwork	0.250
R-free	0.29660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.034
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.660	2.760
High resolution limit [Å]	2.690	2.690
Rpim	0.290	0.890
Number of reflections	133610	37427
<I/σ(I)>	1.71
Completeness [%]	94.4
Redundancy	3.6
CC(1/2)	0.900	0.180

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M MES/Tris (6.5), 10 mM ammonium sulfate, 8-16% PEG8000