8S8U

Escherichia coli translation elongation factor P like protein (EfpL)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.60832
Spacegroup name	P 1 21 1
Unit cell lengths	60.526, 53.341, 64.760
Unit cell angles	90.00, 102.74, 90.00

Refinement procedure

Resolution	59.030 - 2.270
R-factor	0.2473
Rwork	0.246
R-free	0.27470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.890
Data reduction software	autoPROC (1.1.7)
Data scaling software	Aimless (0.7.13)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.160	63.160	2.340
High resolution limit [Å]	2.270	9.060	2.270
Rmerge	0.059	0.045
Rmeas	0.071	0.055	1.000
Rpim	0.040	0.030	0.404
Number of reflections	18764	329	1722
<I/σ(I)>	13.6	29.5	2
Completeness [%]	99.2	98.6	99.2
Redundancy	5.9	5.4	6
CC(1/2)	0.998	0.996	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	100 mM Sodium-HEPES 20% PEG 8000 10 mM Hexaamminecobalt (III) chloride