8S7A
HSPB8acd-C99S/F155T/A156S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 98.273, 98.273, 42.958 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.745 - 1.951 |
| R-factor | 0.2301 |
| Rwork | 0.228 |
| R-free | 0.26580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.707 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.745 | 5.290 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.054 | 4.188 |
| Number of reflections | 7957 | 389 |
| <I/σ(I)> | 19.6 | 0.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.1 | 13.6 |
| CC(1/2) | 1.000 | 0.285 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M lithium sulfate monohydrate 0.1 M Tris pH 8.5 1.26 M ammonium sulfate |
