8S5J
Full-length human cystathionine beta-synthase, basal state, helical reconstruction
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.9 |
| Number of Particle | 749213 |
| Software | cryoSPARC,PHENIX,ISOLDE,UCSF ChimeraX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
