8S4T

DNA bound structure of PrgE from plasmid pCF10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-27
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.8856
Spacegroup name	P 21 21 21
Unit cell lengths	75.776, 90.043, 131.848
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.740 - 2.670
R-factor	0.23052
Rwork	0.229
R-free	0.25257
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.683
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.20.1_4487)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.740	2.765
High resolution limit [Å]	2.670	2.670
Rmeas	0.116	2.067
Number of reflections	26266	2536
<I/σ(I)>	10.71	0.95
Completeness [%]	99.8	99.06
Redundancy	6.7	7
CC(1/2)	0.998	0.628

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15 % Polyethylene glycol 400, 50 mM MES pH 6.5, 80 mM Magnesium acetate, 15 mM Magnesium chloride