8S4J

Structure, substrate selectivity determinants and membrane interactions of a Glutamate-specific TAXI TRAP binding protein from Vibrio cholerae.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537 Angstroms
Spacegroup name	H 3 2
Unit cell lengths	126.660, 126.660, 180.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	69.640 - 1.700
R-factor	0.1914
Rwork	0.190
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.964
Data reduction software	xia2
Data scaling software	xia2
Phasing software	DIMPLE (v2.6.2)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.640	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.064	0.906
Rmeas	0.066	0.206
Rpim	0.015
Number of reflections	61144	6061
<I/σ(I)>	26.31	3.42
Completeness [%]	100.0
Redundancy	20.5
CC(1/2)	1.000	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	2.4 M sodium malonate