8S3C
Crystal structure of Medicago truncatula glutamate dehydrogenase 2 (unliganded)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.977 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 95.651, 164.554, 195.412 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.830 - 2.200 |
R-factor | 0.1742 |
Rwork | 0.174 |
R-free | 0.20370 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.833 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.830 | 2.340 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.087 | 1.764 |
Rmeas | 0.091 | 1.836 |
Number of reflections | 77892 | 12395 |
<I/σ(I)> | 20.68 | 1.63 |
Completeness [%] | 99.8 | 99.2 |
Redundancy | 13.29 | 13.05 |
CC(1/2) | 1.000 | 0.665 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.12 M Ethylene glycols 0.1 M T Sodium HEPES; MOPS (acid) 7.5 20% v/v PEG 500* MME; 10 % w/v PEG 20000 |