8S3C

Crystal structure of Medicago truncatula glutamate dehydrogenase 2 (unliganded)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.977
Spacegroup name	C 2 2 21
Unit cell lengths	95.651, 164.554, 195.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.830 - 2.200
R-factor	0.1742
Rwork	0.174
R-free	0.20370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.833
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.830	2.340
High resolution limit [Å]	2.200	2.200
Rmerge	0.087	1.764
Rmeas	0.091	1.836
Number of reflections	77892	12395
<I/σ(I)>	20.68	1.63
Completeness [%]	99.8	99.2
Redundancy	13.29	13.05
CC(1/2)	1.000	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.12 M Ethylene glycols 0.1 M T Sodium HEPES; MOPS (acid) 7.5 20% v/v PEG 500* MME; 10 % w/v PEG 20000