8S2Y
Crystal structure of alcohol oxidase PaAox1 from Picea abies
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2023-01-21 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.099, 99.273, 87.959 |
| Unit cell angles | 90.00, 100.25, 90.00 |
Refinement procedure
| Resolution | 65.240 - 1.640 |
| Rwork | 0.185 |
| R-free | 0.20960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.771 |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.240 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.119 | |
| Rmeas | 1.802 | |
| Rpim | 0.050 | |
| Number of reflections | 123313 | 13965 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 94.6 | 61.9 |
| Redundancy | 6.3 | |
| CC(1/2) | 0.997 | 0.330 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298.15 | 0.2 M CaCl2 20% PEG 3350 |
