8S2W

SSX structure of Arabidopsis thaliana Pdx1.3 grown in seeded batch conditions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	293
Detector technology	PIXEL
Collection date	2023-01-26
Detector	PSI JUNGFRAU 4M
Wavelength(s)	1.07
Spacegroup name	H 3
Unit cell lengths	177.900, 177.900, 117.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.472 - 2.500
Rwork	0.168
R-free	0.19340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.407
Data reduction software	CrystFEL (0.10.2)
Data scaling software	CrystFEL (0.10.2)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.330	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	47878	4703
<I/σ(I)>	4.4	0.5
Completeness [%]	100.0	100
Redundancy	47.4	45.18
CC(1/2)	0.960	0.170

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7	294	600 mM Sodium citrate and 100 mM HEPES pH 7