8S2V
SSX structure of Lysozyme grown in microfluidic droplets
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2023-01-23 |
Detector | PSI JUNGFRAU 4M |
Wavelength(s) | 1.07 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.900, 78.900, 37.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.853 - 1.800 |
Rwork | 0.146 |
R-free | 0.18970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.767 |
Data reduction software | CrystFEL (0.10.2) |
Data scaling software | CrystFEL (0.10.2) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.900 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 11607 | 663 |
<I/σ(I)> | 17.2 | 0.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 464 | 51.94 |
CC(1/2) | 0.990 | 0.440 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROFLUIDIC | 3 | 294 | 20 mg/mL Lysozyme dissolved in 20 mM sodium acetate pH 4.6 Precipitant: 6% PEG 6000 (W/V), 3.4M NaCl and 1M sodium acetate pH 3.0 |