8S1N

Crystal structure of human Rac1-GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2021-05-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.999872
Spacegroup name	C 2 2 21
Unit cell lengths	41.489, 90.340, 107.405
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.700 - 1.800
R-factor	0.207
Rwork	0.204
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.210
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.700	1.866
High resolution limit [Å]	1.800	1.800
Number of reflections	12342	28
<I/σ(I)>	8.17
Completeness [%]	64.5
Redundancy	1.8
CC(1/2)	0.990	0.310

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.5	100 mM HEPES 7.0, 200 mM Calcium chloride, 20% PEG 6000