8S1M
Crystal structure of human GABARAP fused to EGFR (1076-1099)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-17 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 101.280, 101.280, 101.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.350 - 2.050 |
| R-factor | 0.2055 |
| Rwork | 0.203 |
| R-free | 0.22980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.595 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.350 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 11007 | 798 |
| <I/σ(I)> | 21.91 | 1.01 |
| Completeness [%] | 100.0 | 99.1 |
| Redundancy | 20.1 | 20.1 |
| CC(1/2) | 1.000 | 0.365 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.17 M ammonium acetate 0.085 M sodium citrate 25.5% PEG 4000 15% Glycerol |
