8S0U

Structure of the LytM domain of PrgK from E. faecalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-20
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	53.320, 53.690, 56.350
Unit cell angles	63.30, 70.37, 70.16

Refinement procedure

Resolution	49.130 - 1.500
R-factor	0.1775
Rwork	0.177
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.090
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.130	1.554
High resolution limit [Å]	1.500	1.500
Rmerge	0.085	0.296
Rmeas	0.120	0.419
Number of reflections	76646	7437
<I/σ(I)>	6.98	2.9
Completeness [%]	93.3	90.66
Redundancy	1.9
CC(1/2)	0.911	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	293.15	0.2 M Ammonium sulfate, 0.1 M Sodium acetate, pH 4.6, 30 % w/v PEG 2000 MME