8S0G

Crystal structure of Renilla reniformis luciferase-GFP BRET complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-12
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 32
Unit cell lengths	96.875, 96.875, 361.461
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.437 - 2.378
R-factor	0.2227
Rwork	0.220
R-free	0.28130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.684
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.437	2.460
High resolution limit [Å]	2.378	2.378
Number of reflections	152020	15053
<I/σ(I)>	9
Completeness [%]	99.5
Redundancy	4
CC(1/2)	0.997	0.482

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293.15	sodium tartrate, PEG3350