8S05

ArnAB complex an archaeal ortholog of the Sec23/24 core motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	50.100, 147.400, 62.640
Unit cell angles	90.00, 107.16, 90.00

Refinement procedure

Resolution	47.870 - 2.110
R-factor	0.211
Rwork	0.208
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.095
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.140	2.360
High resolution limit [Å]	2.110	2.110
Rmerge	0.079	0.505
Rmeas	0.095	0.659
Rpim	0.051	0.416
Number of reflections	27478	1375
<I/σ(I)>	9.2	1.8
Completeness [%]	89.9	52.3
Redundancy	3.4	2.4
CC(1/2)	0.996	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.17 mol/L ammonium acetate, 0.085 mol/L sodium citrate pH 6.0, 25.5% (w/v) PEG 4000, 16.5% (v/v) glycerin