8RZZ

Crystal structure of Renilla luciferase RLuc8-GFP BRET complex at pH 9.0 (space group P32)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-12
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 32
Unit cell lengths	97.988, 97.988, 361.996
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.172 - 2.299
R-factor	0.2489
Rwork	0.227
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.563
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.172	2.420
High resolution limit [Å]	2.299	2.299
Number of reflections	172869	25234
<I/σ(I)>	11
Completeness [%]	99.9
Redundancy	10.4
CC(1/2)	0.997	0.572

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293.15	T-mate, Bicine, PEG Smear High, ethylene glycol