8RZG
ZgGH129 from Zobellia galactanivorans soaked with the product of the reaction ADG (3,6-anhydro-D-galactose).
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-25 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98010 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 219.949, 106.825, 165.529 |
| Unit cell angles | 90.00, 113.93, 90.00 |
Refinement procedure
| Resolution | 48.000 - 1.810 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.19390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.799 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.480 | 1.910 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmeas | 0.067 | 1.229 |
| Number of reflections | 272848 | 40731 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 86.5 | 88.5 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.998 | 0.453 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2:1 ratio of 0.14M Na/K-tartrate, 14% PEG 3350 with 1.63 mg/ml of enzyme |
