8RZF
RNase W from Sulfolobus acidocaldarius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 41 |
| Unit cell lengths | 76.230, 76.230, 84.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.300 - 2.040 |
| R-factor | 0.1927 |
| Rwork | 0.192 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.245 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.300 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.040 |
| Number of reflections | 30609 | 3052 |
| <I/σ(I)> | 8 | 1.1 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 4.5 | 4.3 |
| CC(1/2) | 0.900 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | HEPES pH 7.5, MgCl2, polyacrylic acid (5,100) sodium salt |
