8RZ3
Structures of Se- glycosyltransferase SenB from Variovorax paradoxus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2023-10-06 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.898, 56.323, 108.045 |
| Unit cell angles | 90.00, 90.94, 90.00 |
Refinement procedure
| Resolution | 48.620 - 2.150 |
| R-factor | 0.2392 |
| Rwork | 0.237 |
| R-free | 0.28560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.024 |
| Data reduction software | HKL-3000 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.870 | 2.180 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.150 | 0.960 |
| Number of reflections | 71180 | 2762 |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM magnesium sulfate hydrate, 50 mM HEPES sodium pH 7.0, and 1.6 M lithium sulfate monohydrate |
