8RYB
CryoEM structure of M. smegmatis GMP reductase in complex with GMP at pH 7.8, extended conformation.
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.84 |
| Software | RELION,Coot,PHENIX,ISOLDE,SerialEM,MOLREP,UCSF ChimeraX |
| CTF correction | PHASE FLIPPING ONLY |
