8RXO

ACDC domain of Plasmodium falciparum AP2-I transcription factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 41 21 2
Unit cell lengths	78.080, 78.080, 127.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.460 - 3.000
R-factor	0.234
Rwork	0.234
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.738
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	8.860	3.180
High resolution limit [Å]	2.998	6.320	3.000
Rmerge	0.193	0.082	2.533
Rmeas	0.198	0.085	2.592
Number of reflections	8421	610	1303
<I/σ(I)>	12.81
Completeness [%]	99.9
Redundancy	21
CC(1/2)	0.999	0.999	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	1,5 M AmSO4, 0,1 M Sodium Hepes pH 7.5, 2% PEG 400