8RXG

Crystal structure of alpha-keto C-methyl transferase SgvM bound to SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000040
Spacegroup name	P 43 21 2
Unit cell lengths	67.205, 67.205, 183.006
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.090 - 1.813
R-factor	0.1966
Rwork	0.195
R-free	0.23710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.950
Data reduction software	autoPROC
Data scaling software	autoPROC (1.0.5)
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.086	1.844
High resolution limit [Å]	1.813	1.813
Rmerge	0.203	8.708
Rpim	0.059
Number of reflections	39049	1880
<I/σ(I)>	12.6	0.5
Completeness [%]	100.0	100
Redundancy	26.3	27.5
CC(1/2)	0.999	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	100 mM Tris/HCl pH 8.5, 250 mM Li2SO4, and 23% PEG 3350