8RWW
Crystal structure of native alpha-keto C-methyl transferase SgvM bound to ketoleucine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.278, 67.278, 182.967 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.090 - 1.950 |
| R-factor | 0.1837 |
| Rwork | 0.182 |
| R-free | 0.21230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.970 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.090 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.252 | |
| Rmeas | 0.257 | |
| Rpim | 0.050 | |
| Number of reflections | 31639 | 2168 |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 25.8 | |
| CC(1/2) | 0.999 | 0.463 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 20% PEG 3350, 250 mM Li2SO4, and 100 mM Tris/HCl pH 8.5 |
