8RWM
Crystal structure of selenomethionine derivatized alpha keto acid C-methyl-transferases MrsA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97943 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 147.847, 65.531, 74.823 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.820 - 1.644 |
| R-factor | 0.1607 |
| Rwork | 0.160 |
| R-free | 0.18250 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.010 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | HKL2Map |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.820 | 1.730 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.164 | |
| Rmeas | 0.166 | |
| Rpim | 0.023 | 0.332 |
| Number of reflections | 89187 | 12888 |
| <I/σ(I)> | 25.6 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 48.7 | 29 |
| CC(1/2) | 1.000 | 0.777 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100 mM HEPES pH 7.5, 300 mM NaCl, 28% PEG 3350 |
