8RW8

Brain and muscle ARNT-like 1 (BMAL1) PAS-B domain in-complex with core circadian modulator (CCM)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-12
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	30.570, 39.830, 73.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.160
R-factor	0.1974
Rwork	0.196
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.996
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.958
High resolution limit [Å]	1.890	1.890
Number of reflections	7589	721
<I/σ(I)>	6.33
Completeness [%]	99.9
Redundancy	12.7	13.2
CC(1/2)	0.994	0.322

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20 % PEG3350, 0.15 M sodium formate