EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 7.2 |
| Number of Particle | 1462717 |
| Software | crYOLO,RELION,EPU,Gctf,NAMD |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
