8RV3
Structure of the domain IV of the replication factor RctB from Vibrio cholerae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.910, 94.760, 47.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.550 - 2.350 |
| R-factor | 0.1937 |
| Rwork | 0.190 |
| R-free | 0.26700 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.550 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXD |
| Refinement software | BUSTER (2.10.4 (24-FEB-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.550 | 2.398 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.200 | 0.732 |
| Rpim | 0.060 | 0.209 |
| Number of reflections | 11991 | 521 |
| <I/σ(I)> | 13.1 | 3.9 |
| Completeness [%] | 80.8 | |
| Redundancy | 13.1 | |
| CC(1/2) | 0.995 | 0.934 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 200 mM NaI, 20 % PEG 3350 |
