8RUW

Crystal structure of Archaeoglobus fulgidus (S)-3-O-geranylgeranylglyceryl phosphate synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-14
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.03322
Spacegroup name	P 32 2 1
Unit cell lengths	94.891, 94.891, 273.791
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	61.070 - 2.480
R-factor	0.203
Rwork	0.201
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.580
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.260	2.610
High resolution limit [Å]	2.480	2.480
Rmerge	0.196	1.502
Rmeas	0.203	1.561
Rpim	0.055	0.420
Total number of observations	684201	101256
Number of reflections	51921	7472
<I/σ(I)>	9.8	2.4
Completeness [%]	100.0
Redundancy	13.2	13.6
CC(1/2)	0.998	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M (NH4)2SO4 15% w/v PEG 3350 0.1 M Bis-Tris pH 6.5