8RUN
Structure of Oceanobacillus iheyensis group II intron in the presence of Li+, Mg2+, and ARN25850
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-12-05 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.92 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.888, 95.155, 223.768 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.030 - 3.730 |
R-factor | 0.17433 |
Rwork | 0.171 |
R-free | 0.24270 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 2.100 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.030 | 3.868 |
High resolution limit [Å] | 3.730 | 3.735 |
Number of reflections | 20343 | 1962 |
<I/σ(I)> | 16.15 | |
Completeness [%] | 99.7 | |
Redundancy | 13.3 | |
CC(1/2) | 1.000 | 0.602 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 303.15 | 100 mM Mg-Acetate, 200 mM KCl, 50 mM LiCl, 50 mM Na-HEPES pH 7.0, 4% PEG 8000 |