8RUK
Structure of Oceanobacillus iheyensis group II intron in the presence of Na+, Mg2+, and ARN25850
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-05 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.845, 95.144, 225.535 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.550 - 4.810 |
| R-factor | 0.19385 |
| Rwork | 0.191 |
| R-free | 0.24273 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.527 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.550 | 4.982 |
| High resolution limit [Å] | 4.810 | 4.810 |
| Rmerge | 0.140 | |
| Number of reflections | 9771 | 947 |
| <I/σ(I)> | 12.14 | |
| Completeness [%] | 99.8 | |
| Redundancy | 12.5 | |
| CC(1/2) | 0.999 | 0.787 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 303.15 | 100 mM Mg-Acetate, 150 mM NaCl, 50 mM Na-HEPES pH 7.0, 3%PEG 8000 |
