8RUJ

Structure of Oceanobacillus iheyensis group II intron in the presence of K+, Mg2+, 5'-exon, and ARN25850 after 1h soaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-11
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	89.570, 95.098, 227.801
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.520 - 3.120
R-factor	0.19805
Rwork	0.196
R-free	0.23617
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	2.472
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.520	3.236
High resolution limit [Å]	3.120	3.120
Number of reflections	35276	3419
<I/σ(I)>	11.9
Completeness [%]	99.0
Redundancy	13.5
CC(1/2)	0.998	0.316

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		303.15	100 mM magnesium acetate, 200 mM potassium chloride, 10 mM lithium chloride, 50 mM HEPES sodium, pH 7.0, 3% PEG8000