8RUH

Structure of Oceanobacillus iheyensis group II intron in the presence of K+, Mg2+ and 5'-exon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.96
Spacegroup name	P 21 21 21
Unit cell lengths	90.204, 96.484, 231.693
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.230 - 4.550
R-factor	0.18835
Rwork	0.184
R-free	0.25782
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.333
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.230	4.713
High resolution limit [Å]	4.550	4.550
Rmerge	0.054	1.301
Number of reflections	12132	1156
<I/σ(I)>	11.08
Completeness [%]	99.7
Redundancy	6.3
CC(1/2)	0.998	0.573

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		303.15	100 mM magnesium acetate, 100 mM potassium chloride, 10 mM lithium chloride, 50 mM HEPES sodium, pH 7.0, 5% PEG8000