8RU5

ATPase family AAA domain containing 2 with crystallization epitope mutations V1022R:Q1027E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-05
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91739
Spacegroup name	P 21 21 21
Unit cell lengths	38.400, 47.760, 80.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.047 - 1.360
Rwork	0.211
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.546
Data reduction software	xia2
Data scaling software	xia2
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.050	1.400
High resolution limit [Å]	1.360	1.360
Number of reflections	31956	2196
<I/σ(I)>	19.1
Completeness [%]	98.6
Redundancy	6.4
CC(1/2)	0.999	0.519

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG6000 10% ethylene glycol 0.1M tris pH 7.5 0.1M calcium chloride