8RU1
Chromatin remodeling regulator CECR2 with in crystallo disulfide bond
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.910, 77.200, 78.140 |
Unit cell angles | 90.00, 91.22, 90.00 |
Refinement procedure
Resolution | 46.930 - 1.660 |
Rwork | 0.183 |
R-free | 0.21030 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.342 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.930 | 1.700 |
High resolution limit [Å] | 1.660 | 1.660 |
Number of reflections | 61333 | 4508 |
<I/σ(I)> | 18.2 | |
Completeness [%] | 99.5 | |
Redundancy | 3.4 | |
CC(1/2) | 0.999 | 0.768 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 3M sodium chloride 0.1M Bis-Tris pH 5.5 |