8RU1

Chromatin remodeling regulator CECR2 with in crystallo disulfide bond

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	87.910, 77.200, 78.140
Unit cell angles	90.00, 91.22, 90.00

Refinement procedure

Resolution	46.930 - 1.660
Rwork	0.183
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.342
Data reduction software	xia2
Data scaling software	xia2
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.930	1.700
High resolution limit [Å]	1.660	1.660
Number of reflections	61333	4508
<I/σ(I)>	18.2
Completeness [%]	99.5
Redundancy	3.4
CC(1/2)	0.999	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	3M sodium chloride 0.1M Bis-Tris pH 5.5